The other two suspects — Gary Siders Sr. and Christina Siders — also were charged with 16 counts of second-degree child endangerment. A judge entered not guilty pleas on their behalf during a court hearing last week, setting all four defendants's bond at $300,000.
Officials have not confirmed whether all 16 children were related but said they ranged in age from 18 months to 18 years old and included both boys and girls.
Vinton County Prosecutor William Archer clarified that the suspects are not originally from Vinton County.
"This is an intra-family situation. This is not human trafficking. There is nothing to put our other children at risk," Archer said
Earlier last week, Ohio Gov. Mike DeWine said he was being updated on the case.
"It is heartbreaking to learn the conditions that these children were living in and to learn of their medical conditions," he said. "Attorney General Wilson is an experienced prosecutor, and he has told me he has never seen anything like what he saw today. Fran and I pray for these children and thank the children's services workers, law enforcement officers and medical personnel who are helping them."
Attorney General Andy Wilson publicized the case at a news conference last week after the children were removed from the home.
Wilson called them the worst conditions he had encountered in his career, describing it as "pure evil." Several were in serious condition when found, and two were flown to Level 1 trauma centers because of their injuries.
"If they had waited 24 hours, there is a very high probability that we’d be dealing with a death, or multiple deaths, of these children," Wilson said.
Vinton County Sheriff Ryan Cain described horrid conditions the children were in, saying there was a high presence of feces and bacterial matter.
Fox News' Louis Casiano contributed to this report.